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Cimini Lab resources

Below are ways to collaborate our lab, pointers to our software and its documentation, and the educational resources and papers we have been a part of. We hope these resources help you out!

This page last updated 2025-October-17

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About us Work with us Software Educational materials Papers

About us

Lab website

Lab members

Lab paper page

Current lab preprints (from Beth’s CV)

Beth’s Broad bio page with contact email

Beth’s CV

Work with us

Learn about our collaboration models

Image Analysis Collaboration: A Practical Guide

Office hours link (free for anyone! just show up!)

CellProfiler annual support plan

Tool contributions you could make (from Google Summer of Code 2025 proposal)

Attend our semi-annual bootcamp

Software we make

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CellProfiler CellProfiler Analyst Piximi Distributed Something Bilayers

CellProfiler

downloads

manual

plugins documentation

tutorials

video tutorials

examples

GitHub

help forum

pipeline suggester

papers - CellProfiler-plugins [2023] / CellProfiler 4 [2021] / CellProfiler 3 [2018]

CellProfiler Analyst

downloads

manual

GitHub

help forum

papers - CellProfiler Analyst 3 [2021]

Piximi

Piximi.app

manual and tutorials

GitHub

help forum

preprint

video tutorials

Distributed-Something

GitHub

paper

Distributed tools - Distributed-CellProfiler / Distributed-Collate / Distributed-DeepProfiler / Distributed-Fiji / Distributed-HelloWorld / Distributed-OMEZarrCreator

Bilayers

documentation

slide deck describing idea

GitHub

Dockerhub

video tutorial

old demo app

Educational materials

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Educational papers Things we lead Major contributors Minor contributors

Educational papers we wrote

The Twenty Questions of Bioimage Object Analysis[2023] / Tool implementation

A biologist’s guide to planning and performing quantitative bioimaging experiments[2023]

Creating and troubleshooting microscopy analysis workflows: Common challenges and common solutions[2024]

A postdoctoral training program in bioimage analysis[2024]

Things we lead

The Scientific Community Image Forum - Forum.image.sc / Paper - Scientific Community Image Forum: A discussion forum for scientific image software[2019]

Imaging Platform Blog

YouTube tutorials

Office hours link

Ask Erin/Dear Beth image analysis tips video podcast / Resources from podcast episodes

COBA website

Bioimaging Guide - English / Spanish / Portuguese / Czech

The Image Based Profiling Handbook

The Cell Painting Gallery (image repository) - AWS RODA Website / Explore on Quilt / See featured on the National AI Ready Resource (NAIRR) Pilot website / Paper - Cell Painting Gallery: an open resource for image-based profiling[2024]

Bioimage Analysis Beginnings Broad Bootcamp (week-long paid course)

Things we are major (but far from sole) contributors to

Global Bioimage Analysts’ Society (GloBIAS) Global Registries - For bioimage analysts / For bioimage analysis trainers

Virtual I2K Meeting - 2023 edition / 2024 edition / 2025 edition

Things we contributed to (but others led)

Online guide for checklists for imaging and image analysis / Paper - Community-developed checklists for publishing images and image analysis [2023]

Metrics Reloaded online metric chooser / Paper 1 - Metrics reloaded: recommendations for image analysis validation[2024] / Paper 2 - Understanding metric-related pitfalls in image analysis validation[2024]

Microtutor imaging education platform

Boston BioImage Analysis Society (BostonBIAS) - sign up form

Cold Spring Harbor Quantitative Imaging Course (two-week paid course)

Papers you might find useful

Full list of lab papers and preprints

Jump to:

Profiling Tools and methods Reviews Community Surveys Opinions/Perspectives

Profiling protocols and methods

Interpreting image-based profiles using Morpheus and single-cell visualization[2023]

Optimizing the Cell Painting assay for image-based profiling[2023]

A genome-wide atlas of human cell morphology[2024]

Live-cell painting: Image-based profiling in live cells using acridine orange[2025]

Alternate dyes for image-based profiling assays[2025]

Computational tools and methods

ImageJ and CellProfiler: Complements in Open-Source Bioimage Analysis[2020]

Reproducible image-based profiling with Pycytominer[2025]

CellPhePy: A python implementation of the CellPhe toolkit for automated cell phenotyping from microscopy time-lapse videos[2025]

Reviews

Open-source deep-learning software for bioimage segmentation[2021]

Defining the boundaries: challenges and advances in identifying cells in microscopy images[2024]

Community Surveys

2020 BioImage Analysis Survey: Community experiences and needs for the future[2021]

Bridging Imaging Users to Imaging Analysis - A community survey[2023]

2024 survey results - forum post / repository

Opinions/Perspectives

Moving beyond the desktop: Prospects for Practical Bioimage Analysis via the Web[2023]

Building momentum through networks: Bioimaging across the Americas[2024]

The Crucial Role of BioImage Analysts in Scientific Research and Publication[2024]

A Call for FAIR and Open-Access Training Materials to advance Bioimage Analysis (preprint)[2024]

Harmonizing the Generation and Pre-publication Stewardship of FAIR Image Data (preprint)[2024]

Enabling Global Image Data Sharing in the Life Sciences [2025]